How do you measure riboflavin?

How do you measure riboflavin?

Urinary riboflavin: Urinary riboflavin can be measured using high-performance liquid chromatography (HPLC) with fluorometry or competitive protein-binding assays. Rapid and sensitive, the protein-binding assays can be used without first treating the urine sample.

How do you make riboflavin stock solution?

To make Riboflavin stock solution: Dissolve 20 mg of Riboflavin in 5 mL of 0.1 M NaOH and immediately add 50 mL of 0.1 M HCl and make up the solution with DI water to 500 mL. Make working standards by diluting the stock solution with 0.01 M HCl. Store protected from light.

How do you test for riboflavin deficiency?

Diagnosis of Riboflavin Deficiency Riboflavin deficiency should be suspected if characteristic signs develop in a patient with other B vitamin deficiencies. Diagnosis of riboflavin deficiency can be confirmed by a therapeutic trial or laboratory testing, usually by measuring urinary excretion of riboflavin.

Is riboflavin a fluorescence?

Riboflavin exhibits fluorescence due to the pi conjugation and resonance of isoalloxazine and fluoresce in light of wavelength 440 to 500 nm. The intensity of fluorescence is proportional to the concentration of riboflavin in dilute solution (1, 3, 4). The molecular structure of riboflavin is shown in Figure 1.

What is the normal range of riboflavin?

The average daily riboflavin intake from foods and supplements in children and teens is 2.1 mg for ages 2–5 years, 2.2 mg for ages 6–11, and 2.3 mg for ages 12–19. In adults aged 20 and older, the average daily riboflavin intake from foods and supplements is 4.5 mg in men and 4.7 mg in women.

What is riboflavin needed for?

Vitamin B2, also called riboflavin, is one of 8 B vitamins. All B vitamins help the body to convert food (carbohydrates) into fuel (glucose), which is used to produce energy. These B vitamins, often referred to as B-complex vitamins, also help the body metabolize fats and protein.

What is the function of riboflavin?

Riboflavin (vitamin B2) works with the other B vitamins. It is important for body growth. It helps in red blood cell production. It also aids in the release of energy from proteins.

What are the symptoms of low vitamin B2?

The signs and symptoms of riboflavin deficiency (also known as ariboflavinosis) include skin disorders, hyperemia (excess blood) and edema of the mouth and throat, angular stomatitis (lesions at the corners of the mouth), cheilosis (swollen, cracked lips), hair loss, reproductive problems, sore throat, itchy and red …

What diseases are caused by lack of vitamin B2?


  • Angular cheilitis, or cracks at the corners of the mouth.
  • Cracked lips.
  • Dry skin.
  • Inflammation of the lining of the mouth.
  • Inflammation of the tongue.
  • Mouth ulcers.
  • Red lips.
  • Sore throat.

What’s riboflavin good for?

What do u mean by riboflavin?

Vitamin B2, or riboflavin, is one of eight B vitamins that are essential for human health. It can be found in grains, plants, and dairy products. It is crucial for breaking down food components, absorbing other nutrients, and maintaining tissues. Vitamin B2 is a water-soluble vitamin, so it dissolves in water.