What is pRSET vector?
The pRSET vectors are pUC-derived expression vectors designed for high-level protein expression and purification from cloned genes in E. coli. High levels of expression of DNA sequences cloned into the pRSET vectors are made possible by the presence of the T7 promoter.
What is pRSET B?
Plasmid: pRSET-B Bacterial vector for high-level expression of proteins with a cleavable 6xHis tag.
What is pRSET FP?
pRSET/CFP, pRSET/EmGFP and pRSET/BFP vectors are bacterial expression vectors that contain sequences encoding Fluorescent Proteins (FPs). The FP sequences have been cloned into the bacterial expression vector pRSET A to produce pRSET/CFP, pRSET/EmGFP and pRSET/BFP.
What is a pET vector?
pET Bacterial Recombinant Protein Vector. Overview. The pET vector system is a powerful and widely used system for expressing recombinant proteins in E. coli. The gene of interest is cloned into the pET vector under the control of the strong bacteriophage T7 transcription and translation regulatory system.
What is Prseta?
Description. The pRSET vector is designed for high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21(DE3) E. coli. These cells also produce T7 lysozyme to reduce basal expression of target genes.
What are BL21 DE3 cells?
BL21(DE3) is a chemically competent E. coli cell suitable for transformation and high level protein expression using a T7 RNA polymerase-IPTG induction system.
What is EmGFP?
These vectors contain the next-generation EGFP, Emerald Green Fluorescent Protein (EmGFP), which has been further optimized for mammalian expression, as well as Cyan (CFP) and Blue (BFP) Fluorescent Proteins for simple, noninvasive detection of recombinant protein. …
What is Emerald GFP?
Emerald is a basic (constitutively fluorescent) green fluorescent protein published in 1998, derived from Aequorea victoria. It is reported to be a very rapidly-maturing weak dimer.
What are the six different types of vectors?
The detailed explanations on each of these 10 vector types are given below.
- Zero Vector.
- Unit Vector.
- Position Vector.
- Co-initial Vectors.
- Like and Unlike Vectors.
- Co-planar Vectors.
- Collinear Vectors.
- Equal Vectors.
How is the prset vector used for prokaryotic expression?
The pRSET vector is designed for high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced by the production of T7 RNA polymerase in BL21 (DE3) E. coli. These cells also produce T7 lysozyme to reduce basal expression of target genes. The pRSET vector offers:
Can you use a pet vector with a prset vector?
For example, you could try a pET vector with a pRSET vector, which contain different ORI (pBR322 origin and pUC origin, respectively). The only issue is that the pRSET vector is high copy number but pET is not; therefore, you may get significantly more protein expression from pRSET than from pET if you add them into one host cell.
What is the molecular weight of prset / lacZ?
Map of pRSET/lacZ Description pRSET/lacZ is a 5911 bp control vector expressing β-galactosidase. Note that – galactosidase is fused to an N-terminal peptide containing the Xpress™ peptide, 6xHis tag and an enterokinase recognition site. The molecular weight is approximately 120 kDa.
When to use prset a or prset C?
Bacterial vector for high-level expression of proteins with a cleavable 6xHis tag. For other reading frames, use pRSET A or pRSET C. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer.