What is MALDI-TOF mass spectrometry?
MALDI-TOF (matrix-assisted laser desorption/ionization time-of-flight) mass spectrometry is a new technology that has revolutionized pathogen identification and has also proven to accelerate detection of antimicrobial resistance compared to the traditional antibiotic susceptibility tests (AST) as well as DNA …
What is a TOF mass spectrometer used for?
Time of flight mass spectrometry (TOF–MS) is one approach to capturing a broad molecular weight range of signals associated with polar and nonpolar compounds in a single sample. This method uses an electric field to accelerate ions to the same potential.
What does MALDI-TOF MS detect?
Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry (MS) has become a widely used technique for the rapid and accurate identification of bacteria, mycobacteria and certain fungal pathogens in the clinical microbiology laboratory.
Why is TOF used with MALDI?
The type of a mass spectrometer most widely used with MALDI is the time-of-flight mass spectrometer (TOF), mainly due to its large mass range. The TOF measurement procedure is also ideally suited to the MALDI ionization process since the pulsed laser takes individual ‘shots’ rather than working in continuous operation.
How does TOF mass spectrometry work?
Time-of-flight mass spectrometry (TOFMS) is a method of mass spectrometry in which an ion’s mass-to-charge ratio is determined by a time of flight measurement. Ions are accelerated by an electric field of known strength.
What does Q-TOF stand for?
-Mass Time-of-Flight
Agilent Accurate-Mass Time-of-Flight (TOF) and Quadrupole Time-of-Flight (Q-TOF) LC/MS systems. combine Ultra High Definition Q-TOF technology with advanced MassHunter Workstation software—plus the superior HPLC and UHPLC performance and flexibility of the new 1290 Infinity LC.
How does TOF mass spec work?
Time-of-flight mass spectrometry (TOFMS) is a method of mass spectrometry in which an ion’s mass-to-charge ratio is determined by a time of flight measurement. This acceleration results in an ion having the same kinetic energy as any other ion that has the same charge.
What is the principle of MALDI-TOF?
MALDI is the abbreviation for “Matrix Assisted Laser Desorption/Ionization.” The sample for MALDI is uniformly mixed in a large quantity of matrix. The matrix absorbs the ultraviolet light (nitrogen laser light, wavelength 337 nm) and converts it to heat energy.