What is the reaction catalyzed by peroxidase?
The peroxidase enzyme catalyzes the decomposition of hydrogen peroxide in to water and molecular oxygen as illustrate.
What Colour is HRP?
The HRP Color Development Reagent, 4CN (4-chloro-1-naphthol), is used for detect- ing antigens bound to nitrocellulose or other blotting membranes. This substrate will develop an insoluble, purple product on the membrane surface after exposure to horseradish peroxidase conjugated antibodies.
What does TMB substrate do?
TMB substrate is used in ELISA procedures, which utilize horseradish peroxidase conjugates. TMB substrate develops a soluble blue reaction product that can be read at 370 or 655 nm at 370 or 655 nm. The substrate system develops a blue reaction product when reacted with peroxidase.
What is the function of peroxidase in cells?
Peroxidase is an enzyme found in a wide variety of organisms, from plants to humans to bacteria. Its function is to break down hydrogen peroxide (H2O2), which is one of the toxins produced as a byproduct of using oxygen for respiration.
How is HRP redox reaction driven TMB color development?
HRP driven TMB color developmentimmunochemistry.com In Part 2, we take a direct look at the redox reaction mechanism responsible for the conversion of the unoxidized and colorless TMB substrate into a blue-green colored, one electron loss oxidation state product. Special attention is paid to the interactions of the H
Which is the oxidizing substrate in the HRP reaction?
Hydrogen peroxide (H 2 O 2) serves as the oxidizing HRP substrate that initiates the HRP redox cycle. Existing as a covalently linked two hydroxyl chemical structure where the oxygen molecules are linked by a weak (low bond energy) single O-O peroxide or peroxo covalent bond, the presence of this weak O-O peroxide linkage enables it to act as a
Is the HRP catalyzed substrate turnover rate high?
Regarding the performance criteria focusing on HRP catalyzed substrate turnover rate, HRP is considered to be a high substrate turnover rate enzyme [2].
What is the chemical basis of the TMB oxidation process?
Our TMB oxidation process discussion concludes with a more close-up look at the chemical basis behind the acid-stop conversion of the blue green (one electron loss oxidation state) product into the yellow (two electron loss oxidation state) TMB substrate product.