What is lambda phage vector?
Enterobacteria phage λ (lambda phage, coliphage λ) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli. Lambda phage consists of a virus particle including a head (also known as a capsid), tail and tail fibers. The head contains the phage’s double-stranded circular DNA genome.
Is lambda phage a plasmid?
Enterobacteria phage λ (lambda phage, coliphage λ, officially Escherichia virus Lambda) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E. coli)….Lambda phage.
| Escherichia virus Lambda | |
|---|---|
| Genus: | Lambdavirus |
| Species: | Escherichia virus Lambda |
Why is lambda DNA used?
Lambda DNA (48,502 bp) may be used as a molecular weight size marker during nucleic acid gel analysis following digestion with a restriction enzyme (such as HindIII). Lambda DNA can also be used as a substrate in restriction enzyme activity assays.
Can lambda phage be used as cloning vector?
The bacteriophages used for cloning are the λ phage and M13 phage. In replacement vectors, the cleavage sites flank a region containing genes not essential for the lytic cycle, and this region may be deleted and replaced by the DNA insert in the cloning process, and a larger sized DNA of 8–24 kb may be inserted.
What are the advantages of lambda phage vector?
The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector. A number of ingenious modifications help overcome the disadvantages associated with its mode of growth and its size.
Is phage a vector?
Phage vectors consist of an essentially complete phage genome, often M13 phage, into which is inserted DNA encoding the protein or peptide of interest (Figure 1). The remaining genes of the phage vector or helper phage encode other proteins.
What is M13 phage vector?
bacteriophage M13 – filamentous bacteriophage. single-stranded circular DNA genome (6407 bp long) – packaged inside rod-shaped protein capsid.
How long is lambda phage DNA?
48502 bp
Thermo Scientific Lambda is a temperate Escherichia coli bacteriophage. The virion DNA is linear and double-stranded (48502 bp) with 12 bp single-stranded complementary 5′-ends.
Is lambda phage a vector?
Bacteriophage lambda has been in use as a cloning vector for over 25 years, and has been used extensively as an expression vector. The efficiency of packaging and infection, and the simplicity of plaque screening are advantages of lambda as a cloning vector.