When was STR analysis first used?

When was STR analysis first used?

The first STR markers used in forensic casework were selected in 1994 by the Forensic Science Service (FSS) in the United Kingdom for a quadruplex amplification system consisting of four tetranucleotide STRs—TH01, vWA, FES/FPS, and F13A1 (Kimpton et al., 1994).

What is the process of STR analysis?

STR analysis consists of three processes: amplification, electrophoresis, and interpretation. In amplification, extracted DNA is added to chemical reagents and heated, causing the two strands that compose the DNA molecule (they resemble two sides of a “ladder,” as seen in the graphic on page 5) to separate.

What is an STR why is it used in law enforcement?

DNA fingerprinting uses repetitive sequences that are highly variable, called variable number tandem repeats (VNTRs). Modern law enforcement in particular uses short tandem repeats (STRs). DNA fingerprinting can be used to identify a person or to place a person at a crime scene and to help clarify paternity.

How many STR loci are there?

thirteen loci
STR Fact Sheets are available for all thirteen loci (click on locus name for the STR Fact Sheet). For more information, see: Butler, J.M. (2006) Genetics and genomics of core STR loci used in human identity testing.

What are the five steps in RFLP analysis?

RFLP is performed using a series of steps briefly outlined below:

  1. DNA Extraction. To begin with, DNA is extracted from blood, saliva or other samples and purified.
  2. DNA Fragmentation. The purified DNA is digested using restriction endonucleases.
  3. Gel Electrophoresis.
  4. Visualization of Bands.

Is STR non-coding?

Non-coding regions of DNA contain many of the markers used for identity testing. Short tandem repeats (STRs) are sequences of DNA that are 2-6 base pairs long. The number of repeats in STR markers is highly variable among individuals, which makes STRs useful in human identification.

How do you do STR?

The complete process for STR typing includes sample collection, DNA extraction, DNA quantitation, PCR amplification of multiple STR loci, STR allele separation and sizing, STR typing and profile interpretation, and a report of the statistical significance of a match (if observed).

What are the 13 STR loci?

The 13 STR loci CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, and D21S11 have been selected as the core loci for use in CODIS (1).