Where can I buy 2 deoxy D-glucose 2-DG?
|250 mg||In stock||43.00|
|500 mg||In stock||50.00|
|1 g||In stock||59.00|
|1 mL * 10 mM (in DMSO)||In stock||54.00|
What does 2 deoxy D-glucose do?
2-Deoxy- d-glucose is a glucose molecule which has the 2-hydroxyl group replaced by hydrogen, so that it cannot undergo further glycolysis. As such; it acts to competitively inhibit the production of glucose-6-phosphate from glucose at the phosphoglucoisomerase level (step 2 of glycolysis).
Is 2 deoxy D mannose the same as 2 deoxy D-glucose?
Molecular structures of d-glucose and d-mannose. Historically, 2-DG was prepared from d-glucose by the elimination of the hydroxyl group at C-2. However, elimination of the hydroxyl group at C-2 in the d-mannose molecule leads to the same 2-DG compound.
What does 2-deoxyglucose method measure?
Sokoloff and co-workers developed the 2-deoxy-D-[1-14C]glucose (2DG) method in order to study the local cerebral glucose utilization (LCGU) of discrete brain regions in vivo. Energy metabolism of the adult mammalian brain is almost entirely dependent on glucose.
Is 2-deoxyglucose a competitive inhibitor?
Although metrizamide, 2-deoxyglucose, and glucosamine are known to be competitive inhibitors of approximately equal potency for glucose of yeast hexokinase (Ki approximately 0.7 mM for all three), metrizamide is a much weaker competitive inhibitor (Ki about 20 mM) of rat brain hexokinase than either 2-deoxyglucose or …
Does 2-deoxyglucose decrease ATP?
Conversely, 2-deoxyglucose (2DG) blocked glycolysis and partially inhibited glycolytic- dependent oxidative phosphorylation, resulting in a 50% reduction in cellular ATP levels.
How do you make 2 deoxy D glucose?
The preparation method comprises the following steps: taking 3,4,5-tri-O-acetyl D-glucal or 3,4,5-tri-hydroxy-glucal as a raw material, and is placed in a solvent under existence of solid acid, stirring and reacting for certain time under certain temperature, filtering to remove solid acid, washing the solid acid by …
How does 2-deoxyglucose affect ATP?
The glucose analog, 2-deoxyglucose (2DG), inhibits glycolysis, and is predicted to decrease the viability of tumor cells, which are primarily dependent on the glycolytic pathway for ATP generation.
What type of inhibitor is 2-deoxyglucose?
2-Deoxyglucose (2-DG) is best known as an inhibitor of glucose metabolism (1). Inside a cell, it is converted to phosphorylated 2-DG (2-DG-P) by hexokinase, the first and the rate-limiting enzyme in glycolysis. However, 2-DG-P cannot be metabolized by the second enzyme in glycolysis, phosphoglucose isomerase (2).
What happens when glucose is reduced with strong reducing agent like hi and red P?
Glucose on complete reduction with Hl and red P finally gives n -hexane.