What is Giemsa stain used for?
Giemsa stain is performed on paraffin sections. It is used to stain the blood cells of hematopoietic tissues. It can also be applied to all tissue sections in which the presence of microorganisms is suspected. Gram + and Gram Bacteria are not differentiated with this staining.
What parasite do you find in this Giemsa stained blood film?
Screen for and detect spirochetes and blood parasites, including microfilaria, Babesia, Trypanosoma and Plasmodium species.
Can Giemsa stain bacteria?
Giemsa stains the fungus Histoplasma, Chlamydia bacteria, and can be used to identify mast cells.
What is the principle of Grunwald Giemsa stain?
PRINCIPLE: The “neutral” dyes combining the basic dye methylene blue and the acid dye eosin, give a wide color range when staining. The pH of the staining solution is critical and ideally should be adjusted for different fixatives.
How do you make a 10% Giemsa stain?
Make up a 10% Giemsa solution with distilled/deionized water buffered to pH 7.2. If only one slide is to be stained, you will require about 3 ml of prepared stain. Allow 3 drops of stock Giemsa solution (from the Pasteur pipette) to each millilitre of buffered water to give a 10% solution.
What staining reagents are commonly used for examination of parasites in blood film?
Giemsa stain Recommended for detection and identification of blood parasites.
Which parasites can be detected in blood?
The most commonly encountered blood parasites include Haemoproteus spp., Leucocytozoon spp., Trypanosoma spp., Plasmodium spp. (malaria) and microfilaria.
Is microfilaria a parasite?
The microfilaria (plural microfilariae, sometimes abbreviated mf) is an early stage in the life cycle of certain parasitic nematodes in the family Onchocercidae. In these species, the adults live in a tissue or the circulatory system of vertebrates (the “definitive hosts”).
What does Giemsa bind to?
4.1 Giemsa Staining Giemsa is a visible light dye that binds to DNA through intercalation and thus, is used for chromosome staining. It is a mixture of cationic thiazine dyes, most importantly azure B, and anionic eosin dyes such as eosin Y (figure 4.1) [16].
How do you prepare Giemsa for staining?
Popular Answers (1)
- Dissolve 3.8g of Giemsa powder into 250ml of methanol.
- Heat the solution from step 1 to ~60oC.
- Slowly add in 250ml of glycerin to the solution from step 2.
- Filter the solution from step 3.
- The solution needs to stand a period of time prior to use.
What is the purpose of the Giemsa stain?
Giemsa stain is a gold standard staining technique that is used for both thin and thick smears to examine blood for malaria parasites, a routine check-up for other blood parasites and to morphologically differentiate the nuclear and cytoplasm of Erythrocytes, leucocytes and Platelets and parasites.
When to use Wright-Giemsa stain for malaria?
Laboratory diagnosis of malaria Wright (Wright-Giemsa stain Used in hematology, this stain is not optimal for blood parasites. It can be used if rapid results are needed, but should be followed up when possible with a confirmatory Giemsa stain, so that Schüffner’s dots can be demonstrated.
How to prepare a Giemsa stain for a tick smear?
Giemsa stain procedure for thick smear (Blood) For tick smear, prepare a 1:50 ratio of Giemsa stain by mixing 1 ml of stock solution of Giemsa stain to 49 ml of phosphate buffer solution. Take a clean grease-free glass slide and make a thick smear of the blood sample or specimen on it and allow to air dry for 1 hour.
What kind of stain is used for blood parasites?
Wright (Wright-Giemsa) stain Used in hematology, this stain is not optimal for blood parasites. It can be used if rapid results are needed, but should be followed up when possible with a confirmatory Giemsa stain, so that Schüffner’s dots can be demonstrated.