What is a PD-10 column?

What is a PD-10 column?

Overview. PD-10 Desalting Columns contain Sephadex G-25 resin for rapid buffer exchange, desalting, and removal of small contaminants (salts, dyes, radioactive labels) from samples using gravity flow or centrifugation.

How does PD-10 column work?

PD-10 Desalting Columns contain Sephadex G-25 Medium, which allows rapid group separation of high molecular weight substances from low molecular weight substances. PD-10 Desalting Columns are used for desalting, buffer exchange and sample clean up.

How does a desalting column work?

Desalting Column Uses

  1. Removal of salts.
  2. Removal of low molecular weight molecules, for example cofactors, inhibitors, and contaminants.
  3. Buffer exchange.
  4. Stopping reactions between macromolecules and low molecular weight reactants.
  5. Removal of unreacted radioisotope from a sample.

Are PD 10 columns reusable?

Excellent for desalting and buffer-exchange of proteins in small volumes; can be reused without loss of efficiency, even though the pre-packed columns are labeled as disposable.

Can you reuse a desalting column?

Can you reuse PD10 column?

Since PD10 columns are just Sephadex G25 Med in a convenient column, the protocol for cleaning Sephadex G25 can allow you to reuse them multiple times. PD10 can be used many times even without cleaning.

What is the function of desalter?

The purpose of a desalting system is to reduce the salt content of the treated oil to acceptable levels. When the salinity of the produced brine is not too high, merely ensuring that there is a low fraction of water in the oil can reduce salt content.

Why do we do buffer exchange?

Buffer exchange is used to transfer a protein solution into a buffer system appropriate for downstream applications such as ion exchange, electrophoresis or affinity chromatography.