What is the concentration of IgG in rabbit serum?
5 to 12 mg/ml
The concentration of IgG in normal rabbit serum ranges from 5 to 12 mg/ml. The assay measures rabbit IgG in the 0.1-100 ng/ml range. Samples giving rabbit IgG levels above 100 ng/ml should be diluted in blocking buffer before use. A 1:1,000,000 to 1:10,000,000 dilution for serum is suggested for best results.
What is the concentration of IgG in blood?
Specific laboratory determinations The manufacturer indicates the following reference intervals for healthy adults: IgA 70–400 mg/dl, IgG 700–1600 mg/dl and IgM 40–230 mg/dl .
What is the concentration of IgG in human serum?
In normal serum, about 80% is immunoglobulin G (IgG), 15% is immunoglobulin A (IgA), 5% is immunoglobulin M (IgM), 0.2% is immunoglobulin D (IgD), and a trace is immunoglobulin E (IgE).
Is IgG most abundant in serum?
Immunoglobulin G (IgG) is the most abundant antibody isotype in serum. It makes up 80% of the serum antibody levels and is the only one that can cross the placenta. It activates the complement cascade through the classical pathway.
How is IgG concentration calculated?
The concentration of IgG in solution can be determined by substituting the molecular weight, extinction coefficient and λmax into a derived form of the Beer-Lambert Law. A substance’s λmax is the wavelength at which it experiences the strongest absorbance. For IgG, this wavelength is 280 nm.
How much IgG is in human plasma?
IgG is the most abundant immunoglobulin in plasma, found at a concentration of 8 to 18 mg/ml.
How do you quantify antibody concentration?
The concentration of pure antibodies can be estimated from the measured absorbance at 280nm, assuming a value of 13 to 14 for the absorbance of a 1% (10mg/mL) solution in saline or PBS.
What is antibody concentration?
“Antibody concentration” and “antibody titer” have different meanings: Concentration—the total amount of antibody (protein) in solution without regard to function. Titer—the functional concentration or dilution-factor of a stock solution of antibody for a given immunoassay.