Can 10% glycerol be autoclaved?

Can 10% glycerol be autoclaved?

Yes, I have sterilised glycerol in the autoclave in a glassware. It works fine!

How do you sterilize glycerol?

1. Sterilize glycerol by autoclaving for 20 minutes at 15 pounds per square inch (psi) (1.05 kg/cm2) on liquid cycle.
2. To 1.5 ml of bacterial culture, add 0.5 ml of the sterile glycerol in a labeled storage tube (final glycerol concentration of 15%).
3. Vortex the culture to ensure that the glycerol is evenly dispersed.

Do I need to sterilize glycerol?

All Answers (5) There is no problem in sterilizing glycerol in autoclave in a glassware. For freezing, I make a solution of 70% Glycerol, autoclave it and then mix it 1:1 with the fluid present in the culture that I am going to freeze.

How do you prepare 10 glycerol?

To prepare a 10% (v/v) solution: Dilute 1 volume of molecular-biology-grade glycerol in 9 volumes of sterile pure H2O. Sterilize the solution by passing it through a pre-rinsed 0.22-μm filter. Store in 200-mL aliquots at 4°C.

How do you prepare sterile 50% glycerol?

Dilute pure glycerol in distilled water to create a 50% glycerol solution. Use a sterile pipette to measure out 10 mL of both liquids and combine them in a single flask. Stir or shake the flask thoroughly until the liquids are evenly mixed.

How do you revive bacteria from glycerol stock?

To recover bacteria from your glycerol stock, open the tube and use a sterile loop, toothpick or pipette tip to scrape some of the frozen bacteria off of the top. Do not let the glycerol stock unthaw! Streak the bacteria onto an LB agar plate. Grow your bacteria overnight at the appropriate temperature.

Can I use glycerol instead of glycerin?

Glycerin is the commercial name of glycerol which contains 95% of glycerol in it. Glycerin cannot be used interchangeably with glycerol. Glycerin is a less pure form of glycerol. The chemical formula of glycerol and glycerin is the same but the nomenclature is different.

How long does pure glycerine last?

Keep in mind that the average shelf life of glycerine-based extracts is about a year whereas alcohol-based extracts have a shelf life of 4-6 years.

How do you make a 30% glycerol solution?

Prepare a solution of 30% glycerol (v/v) by mixing 30 ml of glycerol with 70 ml of water. Transfer the solution to a screw cap glass bottle and sterilize by autoclaving at 121°C for 15 min.

How much glycerol should I use for sterilization?

Sambrook suggests your glycerol content should be about 15%. I make a 30% Glycerol-LB solution and autoclave it in a glass bottle, and then use a 1:1 dilution (eg. 500ul bacterial culture and 500ul 30%LB-glycerol) to get my final 15%. Works a treat!

What does 10% glycerol in water refer to?

I was surprised to find that the 10% glycerol solution (by weight, not volume) referred to plasma and that the densities for other blood products required increasing amounts of glycerol.

Is it possible to sterilize pure glycerol in autoclave?

In this link explain the problem autoclaving pure glycerol. There is no problem is sterilizing glycerol in autoclave for 15-20 mins. Mix 1:1 ratio of glycerol and your 24 hrs bacterial sample and keep it fridge. Generally sub-culturing has to be done after 13-18 months later (But many have revived the cultures even after 3 yrs also.)

Can you use 10% glycerol as a substitute for blood?

It would be reasonable to say that the common practice has historically been to use a 10% glycerol in water (by weight) mixture as a substitute for blood for various reasons, including transport cooler validation.