How much does a NextSeq cost?
The NEXTSeq 500 will cost $250,000 and the HiSeq X Ten must be purchased in sets of 10 at $10 million for a full set. According to Illumina, the HiSeq X Ten will yield whole human genome sequences for $1,000 each and will have the capability to generate around 15,000-20,000 genomes per year.
How much does a NextSeq 550 cost?
Service price list for external clients
| Instrument | Kit type | Sequencing kit cost |
|---|---|---|
| NextSeq 550 | Mid output | $1,144.75 |
| NextSeq 550 | Mid output | $1,843.00 |
| NextSeq 550 | High output | $1,567.50 |
| NextSeq 550 | High output | $3,002.00 |
How many reads in a NextSeq?
Reads Passing Filter
| NextSeq 550 System High-Output Kit | NextSeq 550 System Mid-Output Kit | |
|---|---|---|
| Single Reads | Up to 400 Million | Up to 130 Million |
| Paired-End Reads | Up to 800 Million | Up to 260 Million |
What NextSeq 500?
The Illumina NextSeq 500 System is a desktop sequencer with power and flexibility to carry out applications such as whole genome sequencing, exome sequencing, whole transcriptome sequencing, mRNA-Seq, and methylation sequencing, among others.
How many lanes does a NextSeq have?
four
How many lanes are on a NextSeq flow cell? The NextSeq flow cell contains four physical lanes. However, libraries are loaded onto the flow cell from a single reservoir. You can sequence a single library or multiple pooled libraries on the flow cell.
What is NextSeq?
The NextSeq 500 System Whole-Genome Sequencing (WGS) Solution enables researchers and clinicians to explore the entire genome of any species cost-effectively for a deeper understanding of biology. The NextSeq 500 System’s simple workflow delivers highly accurate sequencing data.
How much is a NextSeq 2000?
The NextSeq 2000 will have a maximum sequencing data output of 300 Gb and a US list price of $335,000 while the NextSeq 1000 will have a maximum output of 120 Gb and a US list price of $210,000.
How much does MiniSeq cost?
The cost to prepare a sample for the box will range from $25 to $150, and Illumina promises “the same high resolution and accuracy of other Illumina sequencers,” and it is half the price of the MiniSeq, which runs $50,000.
How many cycles of sequencing are there?
Hi Philip – Illumina protocols usually recommend 8-12 PCR cycles for the final library enrichment, but even 5 cycles can be enough. The sequencing facility usually requires libraries to have concentration of 2-10nM (but they use much less for the actual sequencing, e.g. 2-10pM).
How does Illumina NextSeq work?
The basic principle behind how Illumina sequencing works is fluorophore detection. After annealing, the excess nucleotides are washed away and the fluorophore emissions from each cluster are imaged. In the case of the NextSeq 500, only red and green images are taken.
How many flow cells on a NextSeq?
NextSeq 500 Flow Cell NextSeq 500 system uses a four-lane flow cell.