What is the purpose of the thioglycollate in the obligate anaerobe broth?
Thioglycolate broth is a multipurpose, enrichment, differential medium used primarily to determine the oxygen requirements of microorganisms. Sodium thioglycolate in the medium consumes oxygen and permits the growth of obligate anaerobes.
What is the purpose of the thioglycollate test?
Thioglycolate broth is a multipurpose, enriched, differential medium used primarily to determine the oxygen requirements of microorganisms. It acts as an enrichment broth which is most frequently used in diagnostic bacteriology….Composition of Thioglycollate Broth.
How anaerobic growth is maintained in thioglycolate broth?
Sodium Thioglycollate: Sodium Thioglycollate is a reducing agent which maintains a low oxygen tension by removing molecular oxygen from the environment i.e., it creates anaerobic conditions when it reduces molecular oxygen to water.
Where in the thioglycollate broth would you expect to find an obligate anaerobic microbe growing?
Obligate anaerobes will only grow in the lower areas of the tube. Microaerophiles will grow in a thin layer below the richly-oxygenated layer. Facultative or aerotolerant anaerobes can grow throughout the medium but will primarily grow in the middle of the tube, between the oxygen-rich and oxygen-free zones.
What is the function of thioglycollate in the media?
What is the purpose of Thioglycollate media? To determine the oxygen requirement of a bacteria.
What is the purpose of resazurin in thioglycollate medium?
In Fluid Thioglycollate Medium with indicator, resazurin is an oxidation-reduction indicator, being pink when oxidized (as oxygen is absorbed into the medium) and colorless when reduced.
What is the purpose of using thioglycollate media?
What is the purpose of nutrient broth?
Nutrient Broth is a general purpose medium used for cultivating a broad variety of fastidious and non-fastidious microorganisms with non-exacting nutritional requirements. Peptone and yeast extract provide nitrogenous compounds, vitamin B complex, amino acids and other essential growth nutrients.
Is nutrient broth selective or differential?
Nutrient broth, tryptic soy broth, and brain heart infusion, are all examples of complex media. Media that inhibit the growth of unwanted microorganisms and support the growth of the organism of interest by supplying nutrients and reducing competition are called selective media.
What is the role of resazurin in fluid thioglycollate medium is to indicate the presence of media?
Reazurin indicates the presence of CO2 in the media.