What is GFP excitation?
The GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum.
What activates GFP?
Green fluorescent protein (GFP) is a protein in the jellyfish Aequorea Victoria that exhibits green fluorescence when exposed to light. In the jellyfish, GFP interacts with another protein, called aequorin, which emits blue light when added with calcium.
What is the excitation and emission maxima of EGFP?
As demonstrated in Figure 1, the red-shifted variants, typified by EGFP, have a single excitation peak centered at about 488 nm, with an emission peak wavelength of 509 nm.
What is excitation and emission?
An emission spectrum describes the wavelengths of the spectrum emitted by an energetic object. The excitation spectrum is a range of light wavelengths that add energy to a fluorochrome, causing it to emit wavelengths of light, the emission spectrum2.
What does excitation wavelength mean?
Excitation spectra. A fluorophore is excited most efficiently by light of a particular wavelength. This wavelength is the excitation maximum for the fluorophore. This wavelength is the emission maximum for that fluorophore. The excited fluorophore can also emit light at wavelengths near the emission maximum, as shown.
What is the excitation peak of a GFP?
The wild type like variants have their primary excitation peak centered on 395 nm, with an emission peak at 509 nm while the blue emitting mutants generally have an excitation peak at around 380 nm and an emission peak near 460 nm (Figure 1). Table 1. Excitation and emission data of GFP variants
How big is the emission peak of EGFP?
the red-shifted variants, typified by EGFP, have a single excitation peak centered at about 488 nm, with an emission peak wavelength of 509 nm. The wild type like variants have their primary excitation peak centered on 395 nm, with an emission peak at 509 nm while the blue emitting
What are the excitation peaks of green fluorescent protein?
Wild type GFP has two excitation peaks, a major one at 395 nm (in the long UV range) and a smaller one at 475 nm (blue) and its emission peak at 509 nm (green). For wild type GFP, it has been found that exciting the protein at 395 nm causes fairly rapid quenching of the fluorescence.
What kind of excitation filter is used for eGFP?
Fluorescence was determined using either a 485/20 excitation, 530/25 emission filter set or a 400/30 excitation, 508/20 emission filter set. For EGFP and wtGFP determinations using either filter set, a sensitivity setting of 170 was used.