Which cell culture media is carbon dioxide independent?
A eukaryotic growth medium (Program Development Research Group Basal Growth Medium) was developed for CO2-independent maintenance and propagation of human and nonhuman tumor cell lines representing diverse histologies (e.g., cancers of the brain, colon, lung, ovary, and kidney, as well as leukemia and melanoma).
Why is there CO2 in tissue culture?
CO2 is not a metabolic requirement for cell cultures, its purpose is to dissolve into cell culture medium where a small proportion of it reacts with water to form carbonic acid which in turn interacts with its conjugate base (the dissolved bicarbonate ions in the medium) to control a stable physiological pH through the …
Why is CO2 used in cell culture incubator?
The purpose of a CO2 incubator is to maintain an optimal environment for cell growth, by providing carbon dioxide control in a humidified atmosphere with constant temperature.
Why use CO2 independent media?
CO2 Independent Medium is a non-HEPES proprietary medium used for supporting cell growth for a variety of suspension and adherent mammalian cells such as epithelial, fibroblast, and lymphoid cell lines without a CO2 incubator.
Why are cells grown in 5 CO2?
5% CO2 for the cell culture is due to the concentration of sodium bicarbonate in the culture medium. 5%CO2 is needed to buffer the system to ensure that the normal physiological pH is maintained for optimum cell growth. CO2 will react with water to form carbonate.
What happens to cells without CO2?
Without a CO2 supply, cells are adversely affected within five minutes. (Cells can proliferate without CO2 if the medium is buffered with 25 mM HEPES at pH 7.4, but this environment is viable for no more than 10 hours and is highly dependent upon your cell line and cell concentration.)
Why do we grow cells in 5 CO2?
Why do we use 5% CO2 in cell culture?
5%CO2 is needed to buffer the system to ensure that the normal physiological pH is maintained for optimum cell growth. CO2 will react with water to form carbonate.
Why are buffers used in culture media?
pH Buffers & How pH Buffered Culture Media Works A pH buffer acts as either a weak acid or a weak base to ensure that the media will be somewhat resistant to change in pH. This works because the buffer is capable of donating or accepting hydrogen ions which are responsible for establishing pH.